Are all leucodepleted platelet concentrates equivalent? Comparison of CobeLRS Turbo, haemonetics MCS plus LD, and filtered pooled buffy-coat-derivedplatelets

Background and Objectives: A number of technologies are available for the p roduction of leucocyte-depleted platelet concentrates (PCs). This study com pared the characteristics of PCs prepared by three commonly used techniques . Materials and Methods: In total, fifteen units of leucoyte-depleted PCs p repared by the Cobe LRS Turbo apheresis system, Haemonetics MCS+ with in-li ne filter and filtration of PCs derived from pooled buffy coats (BCs) were transferred into the same standard container. Markers to assess status/acti vation and microvesiculation of platelets as well as platelet injury were m easured. Results: pH was well maintained in all types of PCs, The expressio n of CD62P was higher in Cobe LRS Turbo on day 1 but became equivalent betw een the three methods on day 5. A significant correlation was found between the expression of CD62P on platelet surface and soluble CD62P in the plasm a. The degree of phosphatidylserine (PS) exposure was slightly higher in Co be LRS Turbo and BC-PCs than Haemonetics MCS+ on day 1. However, on day 5 b oth apheresis PC values were higher than BC-PCs. A significant correlation was found between PS exposure and microvesiculation, Conclusion: Leucodeple ted PCs prepared by the three methods were different in terms of storage le sion and microvesiculation. The clinical significance of these findings rem ains to be investigated. Copyright (C) 2000 S. Karger AG, Basel.