The long duration of the start-up period is a major drawback of the anaerob
ic wastewater treatment systems. A better understanding of the factors affe
cting biomass aggregation and adhesion, the two main mechanisms of biomass
retention, could make the start-up more efficient and rapid. The objective
of the present work is to determine the effect of the carbon:nitrogen ratio
on the efficiency of biomass retention in upflow anaerobic filters. For th
is purpose, two substrates of two different C:N ratios (nitrogen limited, N
L, and nitrogen balanced, NE) were considered. In fed-batch operation it wa
s shown that a nitrogen limitation in the very first phase of reactor start
-up seems to enhance appreciably biomass adhesion. A feeding strategy to up
flow anaerobic filters, consisting on maintaining a N limitation during the
first two weeks resulted in a moro efficient biomass retention, with a hig
her specific methanogenic activity. By using this policy, the total methano
genic capacity attained after 4 weeks exceeded that obtained in the system
fed with the balanced substrate from the beginning of the operation by 20%.
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