This study aimed at a more objective evaluation of the specimen after total
mesorectal excision [14]. For this reason, a method yielding a simple stai
ned preparation of the totally excised mesorectum was developed. By postope
rative injection of 10 mi of an ink solution into the A. rectalis superior
of 15 specimens, the arterial mesorectal vascular tree was filled. All spec
imens had been collected by means of total mesorectal excision.
In two specimens, in wich the mesorectal sheath fascia had been injured due
to the surgical manipulation, we observed the leakage of ink from the meso
rectum even during the injection. In three further specimens, some ink leak
age in the form of dots occurred from small opened arterioles after the inj
ection was performed. No ink leakage was observed in the remaining specimen
s. Prior to the ink injection, thirteen specimens were macroscopically test
ed and found intact. Three of the fifteen specimens exhibited minor lesions
of the mesorectum that would not have been detected macroscopically withou
t ink tagging. The comparison of the findings provided by the surgeon with
the histopathological evaluation showed that those specimens in which no in
k leakage occurred had an unimpaired mesorectal sheath fascia. These specim
ens correspond to the complete excision of the mesorectum and the removal o
f the tumor in a cancer-sealed package as long as the circumferential rim o
f the specimen has not been infiltrated by the tumor.