Observation of tetrahydrofolylpolyglutamic acid in bacteria cells by matrix-assisted laser desorption/ionization mass spectrometry

Authors
Arnold, RJ Reilly, JP
Citation
Rj. Arnold et Jp. Reilly, Observation of tetrahydrofolylpolyglutamic acid in bacteria cells by matrix-assisted laser desorption/ionization mass spectrometry, ANALYT BIOC, 281(1), 2000, pp. 45-54
Citations number
29
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ANALYTICAL BIOCHEMISTRY
ISSN journal
00032697 → ACNP
Volume
281
Issue
1
Year of publication
2000
Pages
45 - 54
Database
ISI
SICI code
0003-2697(20000515)281:1<45:OOTAIB>2.0.ZU;2-Q
Abstract
Tetrahydrofolylpolyglutamic acid in whole bacteria cells and cell lysates i s analyzed by matrix-assisted laser desorption/ionization mass spectrometry . The speed, mass information, and tolerance to impurities of this techniqu e make it ideal for monitoring the glutamation levels of folic acid in biol ogical systems. Folylpolyglutamic acid is observed in a few strains of E. c oli and two species of Staphylococcus bacteria. The effects of growth time, growth media, and the addition of methotrexate, a dihydrofolate reductase inhibitor, are also studied. (C) 2000 Academic Press.