Mutations at four distinct regions of the rpoB gene can reduce the susceptibility of Helicobacter pylori to rifamycins

Authors
Heep, M Odenbreit, S Beck, D Decker, J Prohaska, E Rieger, U Lehn, N
Citation
M. Heep et al., Mutations at four distinct regions of the rpoB gene can reduce the susceptibility of Helicobacter pylori to rifamycins, ANTIM AG CH, 44(6), 2000, pp. 1713-1715
Citations number
12
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
44
Issue
6
Year of publication
2000
Pages
1713 - 1715
Database
ISI
SICI code
0066-4804(200006)44:6<1713:MAFDRO>2.0.ZU;2-S
Abstract
The transformation of a panel of rpoB mutations (codons 525 to 586) from ri fampin-resistant donor strains into Helicobacter pylori 2802A confirmed the MICs associated with the respective mutations. RpoB V149X random mutations were generated and induced different levels of resistance, depending on th e replacement amino acid. Mutagenesis of the rpoB gene at codon position 70 1 (R701H) induced low-level resistance.