Interactions of ofloxacin and erythromycin with the multidrug resistance protein (MRP) in MRP-overexpressing human leukemia cells

Authors
Terashi, K Oka, M Soda, H Fukuda, M Kawabata, S Nakatomi, K Shiozawa, K Nakamura, T Tsukamoto, K Noguchi, Y Suenaga, M Tei, C Kohno, S
Citation
K. Terashi et al., Interactions of ofloxacin and erythromycin with the multidrug resistance protein (MRP) in MRP-overexpressing human leukemia cells, ANTIM AG CH, 44(6), 2000, pp. 1697-1700
Citations number
24
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
44
Issue
6
Year of publication
2000
Pages
1697 - 1700
Database
ISI
SICI code
0066-4804(200006)44:6<1697:IOOAEW>2.0.ZU;2-Q
Abstract
To investigate interactions between the multidrug resistance protein (MRP) and antimicrobial agents, we examined the effects of 12 agents on vincristi ne sensitivity and efflux of the calcein acetoxy-methyl ester (calcein-AM) of a MRP substrate in MRP-overexpressing cells. Only ofloxacin and erythrom ycin enhanced sensitivity with increased intracellular vincristine accumula tion and inhibited the calcein-AM efflux. Our findings suggest that the two agents are possible MRP substrates and may competitively inhibit MRP funct ion as a drug efflux pump.