Endocellulase and exocellulase activities of two streptomyces strains isolated from a forest soil

Citation
Ltas. Semedo et al., Endocellulase and exocellulase activities of two streptomyces strains isolated from a forest soil, APPL BIOC B, 84-6, 2000, pp. 267-276
Citations number
38
Categorie Soggetti
Biotecnology & Applied Microbiology","Biochemistry & Biophysics
Journal title
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN journal
02732289 → ACNP
Volume
84-6
Year of publication
2000
Pages
267 - 276
Database
ISI
SICI code
0273-2289(200021)84-6:<267:EAEAOT>2.0.ZU;2-G
Abstract
Two Streptomyces strains, M7a and M23, from a Brazilian forest soil were ev aluated for the cellulase production of their supernatants after growth in a microcrystalline cellulose medium, using carboxymethylcellulose and filte r payer as substrates at different temperatures and pH values. Endoglucanas e and exoglucanase activities were compared to a commercial Trichoderma ree sei cellulase using fluorogenic conjugated substrates. Similar specific act ivities were observed for the enzyme preparations of strain M23 and T. rees ei. For M7a the activities were about seven times higher than those obtaine d for T. reesei. Extracellular or cell-associated cellobiase activities wer e not detected in both strains.