Study of different media for production of penicillin G acylase from Bacillus megaterium ATCC 14945

In this study, several fermentation media were tested for the production of penicillin G acylase (PCA) using Bacillus megaterium. The carbon sources s tudied were glucose and lactose. The nitrogen sources studied were enzymati c casein hydrolysates produced with proteases of different specificities. T he replacement of glucose with cheese whey and the addition of free amino a cids in the PGA production were also tested. The results showed a strong co rrelation between the nitrogen source and enzyme yield and the presence of glucose repression. The highest enzyme concentration achieved was 138 IU/L using casein hydrolyzed with 0.6 L of Alcalase(R) and cheese whey.