Genetic and geographic variation of the mangrove tree Bruguiera in Sri Lanka

Citation
Pd. Abeysinghe et al., Genetic and geographic variation of the mangrove tree Bruguiera in Sri Lanka, AQUATIC BOT, 67(2), 2000, pp. 131-141
Citations number
22
Categorie Soggetti
Aquatic Sciences
Journal title
AQUATIC BOTANY
ISSN journal
03043770 → ACNP
Volume
67
Issue
2
Year of publication
2000
Pages
131 - 141
Database
ISI
SICI code
0304-3770(200006)67:2<131:GAGVOT>2.0.ZU;2-G
Abstract
The variation in two Bruguiera species in populations from different climat ic and macrogeographic regions along the western to southeastern coastal ar ea in Sri Lanka was studied using RAPD (Random Amplified Polymorphic DNA), Intra- and interspecific variation was observed using 20 primers of the 45 primers screened. Some primers showed diagnostic banding patterns and allow ed unambiguous differentiation between B. sexangula and B. gymnorhiza witho ut any indication of hybrid individuals. RAPD markers were also used to pro duce a similarity matrix among individuals of a species. A higher degree of polymorphism was observed within populations of B. gymnorhiza in Rekawa, s ituated in the climatically intermediate (between the wet and dry) zone, th an within populations from the wet zone (Galle and Negombo). RAPD data anal ysis did not reveal a clear relationship between genetic differences and ma crogeographical variation along the western and southern coastal area in Sr i Lanka, but indicated that a small population of B. sexangula was genetica lly different. A more pronounced genetic difference at the population level in B. sexangula than in B. gymnorhiza is suggested. (C) 2000 Elsevier Scie nce B.V. All rights reserved.