Jj. Brophy et al., The steam volatile oil of Wollemia nobilis and its comparison with other members of the Araucariaceae (Agathis and Araucaria), BIOCH SYST, 28(6), 2000, pp. 563-578
The leaf essential oil of Wollemia nobilis (Wollemi Pine) has been investig
ated and compared with other members of the family Araucariaceae. All arauc
aroids examined yielded steam volatile oils in low yields. The oil from Wol
lemia nobilis was composed mainly of (+)-16-kaurene (60%), together with al
pha-pinene (9%) and germacrene-D (8%). Oils from Agathis species endemic to
Australia were high in monoterpenes, in contrast to those isolated from ex
tra-Australian species, The major constituents of A. atropurpurea oil were
phyllocladene (13%) and 16-kaurene (19%), followed by alpha-pinene (8%) and
delta-cadinene (9%). A. microstachya yielded oil in which alpha-pinene (18
%) was the major component; the only other components in excess of 5% were
myrcene (7%), bicyclogermacrene (6%) and delta-cadinene (6%). A. robusta oi
l contained spathulenol (37%) and rimuene (6%). Approximately 40% of the oi
l was unidentified sesquiterpenes. A. australis oil contained 16-kaurene (3
7%), sclarene (5%) and an unidentified oxygenated diterpene K (12%) as majo
r components; the only other compound in excess of 5% was germacrene-D (9%)
. 5,15-Rosadiene (60%), and 16-kaurene (7%) were the major constituents of
A. macrophylla oil. A. moorei Oil was rich in sesquiterpenes, but the only
compounds in excess of 5% were allo-aromadendrene (6%), germacrene-D, delta
-cadinene (10%), an unidentified sesquiterpene (12%) and 16-kaurene (6%). I
n A. ovata oil the most significant compounds were caryophyllene oxide (15%
) and phyllocladene (39%). Araucaria angustifolia contained germacrene-D (9
%) and the diterpenes hibaene (30%) and phyllocladene (20%) as major compon
ents of its essential oil. Oils of A. bidwillii, A. columnaris and ii, cunn
inghamii were all low in mono-and sesquiterpenes and high in diterpenes. In
the first, hibaene (76%) was the major constituent; the second contained h
ibaene (9%), sclarene (6%), luxuriadiene (13-epi-dolabradiene)(23%) and two
unidentified diterpene hydrocarbons (B) (33%) and (E)(10%). In the last, 1
6-kaurene (53%) was the most significant component followed by hibaene (29%
). A. heterophylla was unusual in that over half the oil was made up of the
monoterpenoid alpha-pinene (52%), with phyllocladene (32%) being the only
other compound of significance. alpha-Pinene (18%) was a significant compon
ent of A. hunsteinii oil; sclarene (11%) and germacrene-D (5%) were the onl
y other compounds present in concentrations of more than 5%. A. luxurians o
il was composed of 5,15-rosadiene (30%) and luxuriadiene (13-epi-dolabradie
ne) (66%), previously unreported from natural sources. The major components
of A. montana were phyllocladene (61%) and 16-kaurene (23%). Sclarene (20%
), luxuriadiene (19%) and the unidentified diterpene hydrocarbons (B) (25%)
and (E) (10%) were the most important constituents of A. muelleri oil. A.
scopulorum contained large amounts of 16-alpha-phyllocladanol (41%) as well
as luxuridiene (10%) and delta-cadinene and alpha-copaene, both at 6%. (C)
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