Quantitative determination of Cu-thionein from human fluids with application of solid-phase extraction on covalent affinity chromatography with thiol-disulphide interchange support

The aim of our investigation was to carry out quantitative isolation of Cu- thionein (Cu-Th) in human body fluids (urine, plasma and breast milk) in or der to determine the level of exposure to heavy metals. In the experiment c ovalent affinity chromatography with thiol-disulphide interchange gel (CAC- TDI) was used as a solid phase extraction (SPE) support for preconcentratio n of Cu-thionein (Cu-Th) protein and Cu bonded with MT from water and human fluids samples. The present paper is a continuation of early experiments o n the quantitation of Hg-thionein (Hg-Th), Cd-thionein (Cd-Th) and Zn-thion ein (Zn-Th) in human body fluids such as urine, plasma and breast milk. Cop yright (C) 2000 John Wiley & Sons, Ltd.