Extraction and chromatographic separation methods in pharmacokinetic studies of Stobadine (R) - an indole-related antioxidant and free-radical scavenger

This overview provides comprehensive information on the most relevant resul ts of Stobadine preclinical disposition studies. In order to investigate ph armacokinetic processes of the drug in rats, dogs and in human volunteers, several bioanalytical assays based on radiometric, spectrofluorometric, as well as chromatographic determination methods were developed and implemente d. In small laboratory animals, the drug absorption, distribution, metaboli sm and elimination were investigated by administering H-3-labeled Stobadine . Spectrofluorometry was used alternatively for the determination of cold/u nlabeled Stobadine in extracts of biomaterials sampled from larger animal s pecies. The chromatographic separation methods proved, however, to be the m ost advantageous for determining details of the drug disposition and fate i n the body. Copyright (C) 2000 John Wiley & Sons, Ltd.