Extraction and chromatographic separation methods in pharmacokinetic studies of Stobadine (R) - an indole-related antioxidant and free-radical scavenger
L. Soltes et al., Extraction and chromatographic separation methods in pharmacokinetic studies of Stobadine (R) - an indole-related antioxidant and free-radical scavenger, BIOMED CHRO, 14(3), 2000, pp. 188-201
This overview provides comprehensive information on the most relevant resul
ts of Stobadine preclinical disposition studies. In order to investigate ph
armacokinetic processes of the drug in rats, dogs and in human volunteers,
several bioanalytical assays based on radiometric, spectrofluorometric, as
well as chromatographic determination methods were developed and implemente
d. In small laboratory animals, the drug absorption, distribution, metaboli
sm and elimination were investigated by administering H-3-labeled Stobadine
. Spectrofluorometry was used alternatively for the determination of cold/u
nlabeled Stobadine in extracts of biomaterials sampled from larger animal s
pecies. The chromatographic separation methods proved, however, to be the m
ost advantageous for determining details of the drug disposition and fate i
n the body. Copyright (C) 2000 John Wiley & Sons, Ltd.