New fluorogenic substrate for the first continuous steroid sulfatase assay

Authors
Bilban, M Billich, A Auer, M Nussbaumer, P
Citation
M. Bilban et al., New fluorogenic substrate for the first continuous steroid sulfatase assay, BIOORG MED, 10(9), 2000, pp. 967-969
Citations number
8
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
10
Issue
9
Year of publication
2000
Pages
967 - 969
Database
ISI
SICI code
0960-894X(20000501)10:9<967:NFSFTF>2.0.ZU;2-6
Abstract
The screening for new inhibitors of steroid sulfatase requires an efficient test system. To overcome the shortcomings of the available discontinuous f luorimetric assay, several coumarin-type compounds were investigated as pot ential new substrates. 3,4-Benzocoumarin 7-O-sulfate was found to have appr opriate substrate properties for the establishment of the first direct cont inuous assay of steroid sulfatase. (C) 2000 Elsevier Science Ltd. All right s reserved.