Ak. Panda et al., Influences of yeast extract on specific cellular yield of Ovine growth hormone during fed-batch fermentation of E-coli, BIOPROC ENG, 22(5), 2000, pp. 379-383
In most cases of E. coli high cell density fermentation process, maximizing
cell concentration helps in increasing the volumetric productivity of reco
mbinant proteins usually at the cost of lower specific cellular protein yie
ld. In this report, we describe a process for maintaining the specific cell
ular yield of Ovine growth hormone (oGH) from E, coli by optimal feeding of
yeast extract during high cell density fermentation process. Recombinant o
GH was produced as inclusion bodies in Escherichia coli. Specific cellular
yield of recombinant oGH was maintained by feeding yeast extract along with
glucose during fed-batch fermentation. Glucose to yeast extract ratio of 0
.75 was found to be optimum for maintaining the specific cellular oGH yield
of 66 mg/g of E, coli cells. Continuous feeding of yeast extract along wit
h glucose helped in reducing acetic acid secretion and promoted higher cell
growth during fed-batch fermentation. High cell growth of E. coli and high
specific yield of recombinant oGH thus helped in achieving high volumetric
productivity of the expressed protein. A maximum of 2 g/l of ovine growth
hormone was expressed as inclusion bodies in 12 h of fed-batch fermentation
.