Mt. Park et Gm. Lee, Rapid titer assay of adenovirus containing green fluorescent protein gene using flow cytometric analysis, BIOPROC ENG, 22(5), 2000, pp. 403-406
The plaque assay has been widely used for titration of adenovirus (AdV). Ho
wever, it takes usually 2-3 weeks, so this slow assay often impedes bioproc
ess development of large-scale AdV production. In this study, we developed
a rapid AdV titration assay that can be done within a day. Further, unlike
the plaque assay, this assay does not require a laborious serial dilution o
f samples. This rapid assay can be achieved by using green fluorescent prot
ein (GFP) as a marker gene and flow cytometric analysis. It yields a good c
orrelation between infectious titer of AdV harboring GFP and flow cytometri
c parameters such as average green fluorescence intensity or % of infected
cells. Taken together, this rapid assay will facilitate bioprocess developm
ent for efficient large-scale AdV production.