Single-molecule fluorescence observed with mercury lamp illumination

Authors
Unger, M Kartalov, E Chiu, CS Lester, HA Quake, SR
Citation
M. Unger et al., Single-molecule fluorescence observed with mercury lamp illumination, BIOTECHNIQU, 27(5), 1999, pp. 1008
Citations number
21
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOTECHNIQUES
ISSN journal
07366205 → ACNP
Volume
27
Issue
5
Year of publication
1999
Database
ISI
SICI code
0736-6205(199911)27:5<1008:SFOWML>2.0.ZU;2-5
Abstract
We demonstrate that it is possible to observe single fluorescent molecules using a standard fluorescence microscope with mercury lamp excitation and a n inexpensive cooled charge-coupled device (CCD) camera. With this equipmen t, we have been able to observe single molecules of tetramethylrhodamine, r hodamine 6G, fluorescein isothiocyanate and green fluorescent protein. Immo bilized molecules were observed both in air and in aqueous solution.