Expression of PGF(2 alpha) receptor mRNA in normal, hyperplastic and neoplastic skill

Reverse transcription polymerase chain reaction (RT-PCR) and Northern blot analysis was used to determine the level of expression of prostaglandin F-2 alpha (FP) receptor mRNA in various mouse tissues, including normal, hyper plastic and neoplastic mouse epidermis, Steady-state concentrations of FP r eceptor mRNA were low in normal and hyperplastic epidermis, The response of the epidermis to the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (T PA) was biphasic in that FP receptor mRNA was increased immediately after t reatment, followed by a long-lasting downregulation at later time points. F P receptor mRNA was down-regulated in the majority of papillomas obtained b y the mouse skin carcinogenesis initiation-promotion protocol. In carcinoma s, FP receptor mRNA expression was similar to that in normal epidermis, The steady-state concentration of FP mRNA was inversely correlated with PGF(2 alpha) levels in normal and hyperplastic epidermis and in papillomas, indic ating that FP mRNA expression is regulated by this eicosanoid.