Laboratory procedure to wet-mill 100 g of grain sorghum into six fractions

A small-scale (100 g of grain) procedure was developed to wet-mill grain so rghum into six fractions by modifying the procedure of Eckhoff et al (1996) . The wet-milling process was repeated five times on commercial grain sorgh um, and the mean yield (69.4%) of starch (less than or equal to 0.3% protei n) varied by 0.3%, whereas the yields of fiber, gluten, and germ plus bran fractions varied by 5-6%. The starch fraction accounted for approximate to 95% of that in the grain, while the total solids recovered was 99.0%. Four other samples of grain sorghum gave 92-95% recoveries of starches and 98.2- 99.8% recoveries of total solids. All grain sorghum starches had lightness (L*) values and pasting curves nearly equal to those of a commercial maize starch.