An immunosensor of B factor based on surface plasmon resonance

A novel optical immunosensor based on surface plasmon resonance (SPR) has b een developed for identification of antigen. The sensor is designed on the basis of fixing angle of incidence and measuring the reflected intensities in the wavelength range of 200-800 nm. Molecular self-assembling in solutio n is used to form the sensing membrane on gold substrate. The kinetic proce sses of sensing monolayer formation were studied, Human B factor (Bf), an a ctivator of complement C3, was detected in the concentration range from 0. 02-5 mu g/mL. Under the selected experimental conditions, the sensor has a good sensitivity, repeatability, reversibility and selectivity.