Background and Purpose: Validation of a method for obtaining blood samples
that does not change cortisol or prolactin concentrations yet allows serial
blood samples to be collected from animals under anesthesia, without prior
handling, from freely interacting social groups of small mammals.
Methods: Results from five experiments are reported. Male dwarf hamsters (P
hodopus spp,) were housed in modified home cages under continuous flow of c
ompressed air that could be switched to isoflurane in O-2 vehicle without a
pproaching the cages.
Results: Dwarf hamsters respond to manual restraint with behavioral distres
s and increase in the concentration of the dominant glucocorticoid, cortiso
l, and decrease in prolactin concentration. Both effects are evident within
one minute, In contrast, when this new method was used, neither cortisol n
or prolactin changed in response to repeated sample collection (up to 8 suc
cessive samples at 2 hour intervals), prolonged isoflurane exposure, or sub
stantial blood volume reduction (30%). Prolactin concentration was suppress
ed and cortisol concentration was increased in response to stimuli from oth
er hamsters tested without anesthesia. Suppression of prolactin concentrati
on was graded in response to the degree of stress and equaled the pharmacol
ogic reduction caused by bromocryptine mesylate (50 mu g of CB154 x 3 days)
.
Conclusions: The technique is superior to alternatives for studies of behav
ioral endocrinology of freely interacting small, mammals.