Genomic DNA fingerprinting of Oenococcus oeni strains by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA-PCR

Genetic diversity of 60 Oenococcus oeni strains from different wines was ev aluated by numerical analysis of (i) pulsed-field gel electrophoresis (PFGE ) patterns with endonuclease ApaI and (ii) randomly amplified polymorphic D NA (RAPD)-PCR fingerprints with four oligonucleotide primers. Sixty-two per cent of the strains could be distinguished by PFGE, whereas most strains we re identified by distinct RAPD-PCR profiles and associated according to the geographical origin. Because of its rapidity and reliability, RAPD-PCR app eared to be a suitable method for typing and monitoring O, oeni strains in winemaking.