NUCLEOTIDE-SEQUENCES OF HUMAN-CHROMOSOME-19 SHOWING PARTIAL HOMOLOGY WITH TYPE-1 HUMAN-IMMUNODEFICIENCY-VIRUS

Human genome analysis by hybridization in mild conditions revealed mul tiple sequences partly homologous to the HIV-1 regulatory gene nef. Bl ot hybridization with total human DNA and with the human chromosome 19 gene library demonstrated variable homology of such sequences with ne f. In some cases nef-like elements were adjacent and some of them appe ared to be components of longer repeats. Sequencing of one of the clon ed fragments of human chromosome 19 revealed that, alongside with shor t regions homologous to nef, it includes a region homologous to the en v gene located close to nef in the HIV-1 genome. Approximately 700 nef -like elements were found in human chromosome 19; moreover, there were 16 regions homologous to the nef/env-like element. These sequences we re located in different regions of human chromosome 19. The data obtai ned demonstrated that numerous sequences similar to some regions of ex ogenous HIV-1 are present in the human genome, in particular, in chrom osome 19. Nucleotide sequences partly homologous to nef were also foun d in rodent genomes.