EXPRESSION OF RIBOZYME GENE INTEGRATED IN A DEFECTIVE RECOMBINANT ADENOVIRUS

Authors
ZAKHARCHUK AN DORONIN KK BABURINA TM LAZAREV VN NARODITSKY BS
Citation
An. Zakharchuk et al., EXPRESSION OF RIBOZYME GENE INTEGRATED IN A DEFECTIVE RECOMBINANT ADENOVIRUS, Molecular biology, 31(1), 1997, pp. 69-72
Citations number
26
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
31
Issue
1
Year of publication
1997
Pages
69 - 72
Database
ISI
SICI code
0026-8933(1997)31:1<69:EORGII>2.0.ZU;2-M
Abstract
A defective recombinant adenovirus was constructed that carried the ge ne of a ribozyme cleaving the mRNA for secreted alkaline phosphatase ( SEAP). The ribozyme gene was under control of the regulatory elements of the virus-associated RNA gene of fowl adenovirus CELO. The SEAP gen e was expressed in another defective recombinant adenovirus. Coinfecti on of HeLa cells resulted in a decline in SEAP activity in the culture medium. RT-PCR assays proved specific mRNA cleavage in infected cells .