PROPERTIES OF FREE AND LATENT NATURAL ANTIBODIES REACTING WITH DNA AND CARDIOLIPIN

A study was made of the properties of natural antibodies in gamma-glob ulin preparations from sera of healthy donors. Affinity chromatography yielded two types of natural antibodies, free and latent (revealed up on ion-exchange chromatography on QAE Sephadex). Autoantibodies isolat ed on DNA- and cardiolipin-carrying sorbents proved to be similarly he terogeneous and reacted with the same antigen panel. Free and latent a ntibodies were equally reactive with DNA and bacterial lipopolysacchar ide, but the free ones were much more reactive with phosphatidyl choli ne while the latent ones reacted preferentially with cardiolipin.