Electrochemical detection of secreted alkaline phosphatase: Implications to cell based assays

Authors
Kelso, E McLean, J Cardosi, MF
Citation
E. Kelso et al., Electrochemical detection of secreted alkaline phosphatase: Implications to cell based assays, ELECTROANAL, 12(7), 2000, pp. 490-494
Citations number
15
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ELECTROANALYSIS
ISSN journal
10400397 → ACNP
Volume
12
Issue
7
Year of publication
2000
Pages
490 - 494
Database
ISI
SICI code
1040-0397(200005)12:7<490:EDOSAP>2.0.ZU;2-D
Abstract
A sensitive electrochemical assay method is described for secreted placenta l alkaline phosphatase (SPAP). The enzyme activity is measured using 2-naph thyl phosphate as the electrochemical substrate. The product of the reactio n, 2-naphthol, is detected using Osteryoung square wave voltammetry (OSWV) with good sensitivity and precision using in-house screen printed carbon el ectrode assembly. The assay format is adapted to develop a cell based assay for herpes simplex virus (HSV).