At. Hill et al., Airways inflammation in chronic bronchitis: the effects of smoking and alpha(1)-antitrypsin deficiency, EUR RESP J, 15(5), 2000, pp. 886-890
Airways inflammation in chronic bronchitis is thought predominantly to be a
direct consequence of neutrophil recruitment and release of elastase in re
sponse to factors such as cigarette smoke. The aims of this study were to a
ssess the role of smoking and determine whether the serum elastase inhibito
r alpha(1)-antitrypsin (alpha(1)AT) influenced the process.
Airways inflammation was compared between patients with chronic obstructive
bronchitis with (n=39) and without (n=42) severe alpha(1)AT deficiency. Th
e authors assessed the sputum concentration of the neutrophil chemoattracta
nts interleukin-8 (IL-8) and leukotriene (LT)B-4, myeloperoxidase (MPO) as
a marker of neutrophil influx, neutrophil elastase activity and its natural
inhibitors, alpha(1)AT and secretory leukoprotease inhibitor (SLPI), Final
ly serum alpha(1)AT was measured to determine the degree of protein leakage
(sputum sol serum alpha(1)AT ratio).
Compared to current smokers, the exsmokers had a lower concentration of the
chemoattractant IL-8 (p<0.05) and a lower MPO concentration, although this
failed to reach conventional statistical significance (p=0.06). Patients w
ith alpha(1)AT deficiency had greater inflammation in the larger airways wi
th increased LTB4 (p<0.005), MPO (p<0.001), neutrophil elastase activity (p
<0.01), protein leak (p<0.001), and were found to have a lower anti-protein
ase screen with both reduced sputum (alpha(1)AT (p<0.001) and SLPI concentr
ations (p<0.05).
The reduction in sputum interleukin-8 levels in exsmokers may decrease neut
rophil influx and thus explain the slower rate of neutrophil mediated progr
ession of lung disease compared to subjects who continue to smoke. Patients
with alpha(1)-antitrypsin deficiency had greater inflammation suggesting t
hat alpha(1)-antitrypsin plays an important role in protecting the larger a
irways from the inflammatory effects of elastase activity and may explain t
heir more rapid progression of disease.