Leishmania (Viannia) guyanensis: A new minicircle class exclusive to this specie isolated from a DNA cosmid library useful for taxonomic purposes

Leishmania (Viannia) guyanensis: A new minicircle class exclusive to this s pecie isolated from a DNA cosmid library useful for taxonomic purposes. Exp erimental Parasitology 94, 143-149. In this paper we describe a new minicir cle class exclusive to Leishmania (Viannia) guyanensis. The minicircle clas s was obtained with the aid of a total DNA cosmid library. The library was screened with an EcoRI fragment isolated from L. (V.) guyanensis (M4147). A fter screening seven clones were selected which showed strong hybridisation . Clones were digested and hybridised with the same probe. After hybridisat ion only one clone containing the desired fragment was positive. The fragme nt, sized around 1000 bp was subcloned into pBluescript for sequencing. Seq uence analysis using the GCG programme showed no substantial homology with any sequences previously reported, apart from the expected homology with th e conserved region of Leishmania kDNA sequences. The probe hybridised stron gly only to L. (V.) guyanensis kDNA after medium stringency washing. (C) 20 00 Academic Press.