Gene-centromere mapping of 312 loci in pink salmon by half-tetrad analysis

We estimated recombination rates between 312 loci and their centromeres in gynogenetic diploid pink salmon (Oncorhynchus gorbuscha) that we produced b y initiating development with irradiated sperm and blocking the maternal se cond meiotic division. Amplified fragment length polymorphisms (AFLPs) were significantly more centromeric than loci identified by three other techniq ues (allozymes, microsatellites, and PCR using primer sequences from inters persed nuclear elements). The near absence of AFLPs in distal regions could limit their utility in constructing linkage maps. A large proportion of lo ci had frequency of second division segregation (y) values approaching 1.0, indicating near complete crossover interference on many chromosome arms. A s predicted from models of chromosomal evolution in salmonids based upon re sults with allozyme loci, all duplicated microsatellite loci that shared al leles (isoloci) had y values of nearly 1.0.