A transcript map of a 2-Mb BAC contig in the proximal portion of the mouseX chromosome and regional mapping of the scurfy mutation

A physical clone contig has been constructed, spanning 2 Mb on the proximal mouse X chromosome containing the mouse scurfy (sf) and tattered (Td) muta tions. Extensive transcript mapping in this interval has identified 37 pote ntial transcription units, including a number of novel genes, and 4 pseudog enes. These genes have been ordered by STS content and restriction mapping. Comparison of the transcript map to the corresponding region in human Xp11 .23-p11.22 shows extensive homology, with complete conservation of gene ord er for loci in common between the two maps. Further, using a novel method t o identify simple sequence length polymorphisms, we have developed a number of genetic markers, which has enabled the region containing the sf mutatio n to be narrowed to <300 kb. This contig has already allowed the cloning of the Td gene using a candidate gene approach and now serves as a starting p oint for the cloning of the sf mutation. (C) 2000 Academic Press.