Reversed-phase high-performance liquid chromatographic separation and determination of 4-amino-azobenzene-4 ',5-disulfonic acid and related products in industrial wastewater effluents

A simple and rapid reversed-phase high-performance liquid chromatographic m ethod for the separation and determination of 4-aminoazobenzene-4',5-disulf onic acid (AABDS) and its process-related impurities was developed. The sep aration was achieved on a mu-Bondapak C-18 column using 0.15 M ammonium sul fate-acetonitrile (55:45) (v/v) as eluent, A UV-visible spectrophotometric detector fixed at 386 nm was used both for detection and quantitation. The method was used not only for quality assurance but also for process develop ment and wastewater management of AABDS.