Determination of urinary urate with alkylamine glass-bound uricase

Authors
Pundir, CS Bhargava, AK Gupta, R
Citation
Cs. Pundir et al., Determination of urinary urate with alkylamine glass-bound uricase, I J BIOCH B, 37(2), 2000, pp. 140-142
Citations number
20
Categorie Soggetti
Biochemistry & Biophysics
Journal title
INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
ISSN journal
03011208 → ACNP
Volume
37
Issue
2
Year of publication
2000
Pages
140 - 142
Database
ISI
SICI code
0301-1208(200004)37:2<140:DOUUWA>2.0.ZU;2-O
Abstract
Porcine liver uricase immobilized on alkylamine glass (pore diameter 55 nm) through glutaraldehyde coupling has been found useful in the determination of uric acid in urine. The minimum detection limit was 12.0 mu g/0.1 ml sa mple and the recovery of added uric acid was 91%. The coefficient of variat ion (CV) were <6.0% and <2.0% for within and between batch assay respective ly. A good correlation (r=0.93) was found between urate values obtained by a standard commercial method and the present method.