ISOLATION OF COCCIDIAN ENTEROEPITHELIAL STAGES OF TOXOPLASMA-GONDII FROM THE INTESTINAL-MUCOSA OF CATS BY PERCOLL DENSITY-GRADIENT CENTRIFUGATION

Authors
OMATA Y TAKA A TERADA K KOYAMA T KANDA M SAITO A DUBEY JP
Citation
Y. Omata et al., ISOLATION OF COCCIDIAN ENTEROEPITHELIAL STAGES OF TOXOPLASMA-GONDII FROM THE INTESTINAL-MUCOSA OF CATS BY PERCOLL DENSITY-GRADIENT CENTRIFUGATION, Parasitology research, 83(6), 1997, pp. 574-577
Citations number
17
Categorie Soggetti
Parasitiology
Journal title
Parasitology research → ACNP
ISSN journal
09320113
Volume
83
Issue
6
Year of publication
1997
Pages
574 - 577
Database
ISI
SICI code
0932-0113(1997)83:6<574:IOCESO>2.0.ZU;2-N
Abstract
A method for isolation of enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of experimentally infected cats was develo ped using Percoll density-gradient centrifugation. Gamonts and merozoi tes were obtained essentially free of host-cell debris. A recovery rat e of nearly 30% of the parasites in the original preparations was obta ined by this method. Merozoites were separated from gamonts by filtrat ion through a 3-mu m polycarbonate filter.