Bulleidia extructa gen. nov., sp nov., isolated from the oval cavity

Five strains of anaerobic non-sporing Cram-positive bacilli isolated from a dvanced periodontitis (four strains) and a dentoalveolar abscess tone strai n) that did not correspond to existing species were subjected to phenotypic and genetic characterization. Following 16S rDNA sequence analysis, they w ere found to constitute a novel branch of the low G+C Cram-positive divisio n of the phylogenetic tree related to Erysipelothrix rhusiopathiae and Hold emania filiformis. A new genus Bulleidia, and the species Bulleidia extruct a, are proposed. Growth of B. extructa in broth media was poor but was enha nced by the addition of fructose, glucose or maltose together with Tween 80 . Glucose and maltose were fermented and arginine was hydrolysed. Acetate, lactate and trace amounts of succinate were the end products of glucose fer mentation. The G+C content of the DNA of the type strain is 38 mol%. The ty pe strain of Bulleidia extructa is DSM 13220(T).