Time-resolved small angle scattering: kinetic and structural data from proteins in solution

Authors
Roessle, M Manakova, E Laure, I Nawroth, T Holzinger, J Narayanan, T Bernstorff, S Amenitsch, H Heumann, H
Citation
M. Roessle et al., Time-resolved small angle scattering: kinetic and structural data from proteins in solution, J APPL CRYS, 33(1), 2000, pp. 548-551
Citations number
12
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF APPLIED CRYSTALLOGRAPHY
ISSN journal
00218898 → ACNP
Volume
33
Issue
1
Year of publication
2000
Part
3
Pages
548 - 551
Database
ISI
SICI code
0021-8898(20000601)33:1<548:TSASKA>2.0.ZU;2-8
Abstract
Using the high flux of a third generation synchrotron sources at ESRF and E LETTRA, structural changes of the chaperonin system GroE were analyzed by t ime-resolved small angle scattering. A fast mixing device were established which permits determination of the solution scattering parameters of the E. coli chaperonin system GroE in time slices of 300msec. Using this device, the reaction pathway of the two component system, GroEL and GroES was analy zed.