Solution structure of detergent micelles at conditions relevant to membrane protein crystallization

In this study small angle neutron scattering was used to characterize the f ormation of micelles in aqueous solutions of the detergents DMG and SPC as a function of detergent concentration and ionic strength of the solvent. Th e effects on the micelle structure of the additives glycerol and PEG, alone as well as in combination typical for membrane protein crystallization, we re also explored. This study suggests that the micelles are rod-like in for m at the concentrations studied. The size of the micelles was observed to i ncrease with increasing ionic strength but decrease with the addition of gl ycerol or PEG.