S. Perrin et al., Effects of fructooligosaccharides and their monomeric components on bile salt resistance in three species of bifidobacteria, J APPL MICR, 88(6), 2000, pp. 968-974
The influence of fructooligosaccharides (FOS) and their monomeric component
s on bile salt resistance of Bifidobacterium breve ATCC 15700, Bif. longum
ATCC 15707 and Bif. animalis ATCC 25527 was examined. The neosugars induced
fructofuranosidase activities for the degradation of these saccharides. Fo
r the three strains tested the growth was identical and bile salts had the
same inhibitory effect on growth whatever the carbohydrate used. The surviv
al of Bif. breve and Bif. longum, in the presence of glycodeoxycholic acid
depended, however, on carbohydrates: the toxic effects of the bile salt cou
ld be partly alleviated by the addition of a metabolizable C-source. For Bi
f. animalis, the presence of any carbohydrate in the incubation medium did
not enhance the viability of the strain. But in the three deconjugating str
ains of bifidobacteria studied, the presence of neosugar during the growth
led to improved resistance to the bactericidal effect of the bile salt comp
ared with the monomeric components of these neosugars (glucose and fructose
).