Saxiphilin, a saxitoxin-binding protein with two thyroglobulin type 1 domains, is an inhibitor of papain-like cysteine proteinases

The type 1 domain of thyroglobulin is a protein module (Thyr-1) that occurs in a variety of secreted and membrane proteins. Several examples of Thyr-1 modules have been previously identified as inhibitors of the papain family of cysteine proteinases, Saxiphilin is a neurotoxin-binding protein from b ullfrog and a homolog of transferrin with a pair of such Thyr-1 modules loc ated in the N-lobe. Saxiphilin is now characterized as a potent inhibitor o f three cysteine proteinases as follows: papain, human cathepsin B, and cat hepsin L, The stoichiometry of enzyme inhibition reveals that both Thyr-1 d omains of saxiphilin inhibit papain (apparent K-i = 1.72 naa), but only one of these domains inhibits cathepsin B (K-i = 1.67 nM) and cathepsin L (K-i = 0.02 nM). Physical association of saxiphilin and papain blocked from tur nover at the active-site cysteine residue can be detected by cross-linking with glutaraldehyde. The rate of association of saxiphilin and cathepsin B is strongly pH-dependent with an optimum at pH 5.2, reflecting control by a t least two H+-titratable groups. These results further demonstrate that va rious Thyr-1 domains are selective inhibitors of cysteine proteinases with utility in the study of protein interactions and degradation.