Truncated activin type II receptor inhibits erythroid differentiation in K562 cells

Two receptor serine/threonine kinases (types I and II) have been identified as signaling transducing, activin receptors. We studied the possibility of inhibiting activin A-dependent differentiation in K562 cells, using a domi nant negative mutant of type II receptor. A vector was constructed expressi ng activin type II truncated receptor (ActRIIa) that lacks the cytoplasmic kinase domain. Since activin type I and II receptors form heteromeric compl exes for signaling, the mutant receptors compete for binding to endogenous receptors, hence acting in a dominant negative fash ion. K562 cells were st ably transfected with ActRIIa, and independent clones were expanded. The tr uncated cDNA was integrated into the genome of the transfectants, as shown by polymerase chain reaction; and the surface expression of truncated recep tors was shown by affinity cross-linking with I-125-activin A. In wild-type K562 cells, activin A induced erythroid differentiation and cells started to express hemoglobins. In transfected cells expressing ActRIIa, the induct ion of erythroid differentiation was abrogated and less than 10% of cells w ere hemoglobin-containing cells after culture with activin A. Further trans fection with wild-type type II receptors rescued the mutant phenotype of th ese transfectants, indicating that the effect of ActRIIa is dominant negati ve. In addition, phosphorylation of the cytoplasmic kinase domain of the ty pe II receptor in vitro confirms the autophosphorylation of this portion of the receptor. Therefore, induction of erythroid differentiation in vitro i s mediated through the cell surface activin receptor, and interference with this receptor signaling inhibits this process of differentiation in K562 c ells. (C) 2000 wiley-Liss, Inc.