Synergistic activity of capelin protein hydrolysates with synthetic antioxidants in a model system

Capelin protein hydrolysates (CPHs) were examined in a beta-carotene-linole ate model system together with synthetic antioxidants, namely butylated hyd roxyanisole (BHA), butylated hydroxytoluene (BHT), and tert-butyl hydroquin one (TBHQ). Addition of CPHs was at 0.25 and 1 mg per 5 mL in the above emu lsion. BHA and BHT were added at levels of 2.5 and 5 mu g and TBHQ at 5 and 10 mu g. Absorbance of model emulsion system at 470 nm was recorded every 30 min for 2 h. CHPs and synthetic antioxidants inhibited oxidation of lino leic acid effectively. In the Experiment with BHA, the influence of CPHs wa r significant (p<0.01) for the samples incubated for 60, 90, and 120 min; a nd in experiments with BHT and TBHQ during the entire incubation period. Th e influence of added BHA and BHT on inhibition of bleaching of beta-caroten e in the emulsion system was significant (p <0.01) during the incubation pe riod and after 60, 90 and 120 min when TBHQ was used. A synergistic effect was observed only for CPHs and TBHQ when incubation time was 60, 90, and 12 0 min.