Countercurrent chromatographic separation of vitamins by cross-axis coil planet centrifuge with eccentric coil assemblies

Vitamins were resolved by high-speed countercurrent chromatography using a cross-axis coil planet centrifuge equipped with eccentric coil assemblies. A two-phase solvent system composed of 1-butanol/ethanol/aqueous 0.15 M mon obasic potassium phosphate (8:3:8) was used for the separation of water-sol uble vitamins such as thiamine nitrate, pyridoxine hydrochloride, and nicot inamide, while 2,2,4-trimethyl pentane (isooctane)/methanol (1:1) was used for the separation of fat-soluble vitamins such as calciferol, vitamin A ac etate, and (+/-)-alpha-tocopherol acetate. Overall results of experiments r evealed that the cross-axis coil planet centrifuge is useful for the separa tion of vitamins by selecting suitable two-phase solvent systems.