Phylogenetic relationships among 8 subspecies of Sigmodon hispidus from Nor
th, Central, and South America were examined using DNA-sequence data from t
he mitochondrial cytochrome-b gene. The magnitude of DNA-sequence variation
detected among these subspecies, in some cases, exceeded levels of intersp
ecific variation observed among currently recognized species of Sigmodon. T
hese data suggest that S. hispidus is paraphyletic and that a minimum of 3
species exist within the currently recognized taxon. Consequently, the taxo
nomy should be revised to recognize the following samples as distinct speci
es: those from the United States and north-central Mexico, those from south
ern Mexico, and those from Central and South America.