Biosensor analysis of the interaction between immobilized human serum albumin and drug compounds for prediction of human serum albumin binding levels

The interactions between a set of drugs, selected on the basis of reported human serum albumin (HSA) binding levels, and immobilized HSA were investig ated using surface plasmon resonance technology. Major HSA binding sites we re available after immobilization. The intensity of the signal obtained fro m the interaction of the drug with the HSA surface was correlated with the reported HSA binding level. Drugs were classified into groups corresponding to high, medium, or low HSA binding based on the injection of the drug at 80 mu M concentration. A set of 10 drugs binding to alpha(1)-acid glycoprot ein (AGP) was also investigated and correlated with reported AGP binding da ta. The throughput of the presented assay is 100 compounds/24 h, and the sa mple consumption is less than 100 mu L (8 nmol).