El. Stern et al., Spatiotemporal induction patterns of cytokine and related immune signal molecule mRNAs in response to intrastriatal injection of lipopolysaccharide, J NEUROIMM, 106(1-2), 2000, pp. 114-129
The brain's response to a direct immune challenge was examined by in situ h
ybridization histochemistry. Lipopolysaccharide (bacterial endotoxin) injec
ted acutely into rat striatum induced mRNA expression for inhibitory factor
kappa B alpha, interleukin (IL)-1 beta, tumor necrosis facror-alpha, IL-6,
IL-12 p35, inducible nitric oxide synthase, IL-1 receptor antagonist, and
the type 1 IL-1 receptor. Expression patterns were evaluated at select time
points ranging from 15 min to 3 days post-injection. Rats injected with ve
hicle alone were used to control for mechanical effects. Following lipopoly
saccharide administration, a wave of mRNA induction within brain parenchyma
radiated outward from the injection site, generally peaking in intensity a
t the 16-h time point. The individual profiles of cytokine mRNA induction p
atterns reveal that the brain's immune response to local inflammatory stimu
lation is quite elaborate and in many ways resembles the progression of cyt
okine induction customary of localized inflammation in peripheral tissues.
(C) 2000 Elsevier Science B.V. All rights reserved.