Hormonal regulation of lipogenesis in cultured adipose tissue from perinatal and month-old goats

Perinatal (1-2 days old) and month-old (24-32 days old) male goats were use d to investigate the effect of age and chronic culture (24 h) of perirenal and omental adipose tissue explants in the presence of insulin, cortisol an d recombinant bovine somatotrophin (bGH), alone or in different combination s, on lipogenic responses in the absence and in the presence of catecholami nes in acute incubations (2 h). Addition of insulin alone or bGH alone or i nsulin plus bGH had no effect on the rate of fatty acid synthesis in tissue s of both groups. The presence of cortisol alone decreased (P < 0.05) and t he combination of insulin plus cortisol increased (P < 0.05) lipogenesis in perirenal adipose tissue explants from month-old goats. No such effects of these hormonal combinations were noted in omental adipose tissue from peri natal and month-old gents or in perirenal adipose tissue from perinatal goa ts. When perirenal adipose tissue explants from month-old goats were cultur ed in the presence of insulin plus cortisol plus bGH the rates of lipogenes is were lower (P < 0.05) than those in cultures with insulin plus cortisol. Isoprenaline (Iso), but not noradrenaline (Ne), inhibited lipogenesis in f reshly prepared adipose tissue explants; however, when maintained in tissue culture for 24 h, a response to Ne was induced. In cultured perirenal adip ose tissue explants of both perinatal and month-old goats, the mean values of fatty acid synthesis were decreased (P < 0.05) by Ne and Iso: Ne and Iso were equally effective. However, in cultured omental adipose tissue explan ts, Iso was mole effective than Ne. (C) 2000 Elsevier Science B.V. All righ ts reserved.