Zen diploperennis is a perennial Mexican teosinte with favourable clonal pr
opagation characteristics. Being a species of che genus Zen it is used in m
aize breeding programs as a source of virus' resistance and other genetic t
raits, including regeneration capacity. Determination of optimal growth con
ditions of this species is important from the perspective of its potential
as a forage crop as well as its potential use in breeding programs and gene
tic engineering. The responses of 2-month old Z. diploperennis plants to ni
trate availability were investigated. Significant differences have been fou
nd in the growth, CO2 exchange (photosynthesis, respiration), chlorophyll a
nd nitrogen concentration between plants grown at high (HN) and low (LN) ni
trate supply. At HN, the plants grew faster than those cultivated at LN. Ra
tes of photosynthesis, leaf respiration, root respiration (including the no
nphosphorylating alternative pathway), quantum yield, photosynthetic nitrog
en-use efficiency, concentration of chlorophylls and some growth parameters
(total biomass, specific leaf area, leaf mass ratio and shoot mass ratio)
were higher for the plants at HN. At both HN and LN, we found relatively hi
gh values of PNUE.