An RNA helicase, RHIV-1, induced by porcine reproductive and respiratory syndrome virus (PRRSV) is mapped on porcine chromosome 10q13

The impact of porcine reproductive and respiratory syndrome virus (PRRSV) i nfection on porcine alveolar macrophages (Mo) was examined by differential display reverse transcription PCR (DDRT-PCR). A PRRSV-induced expressed gen e tag (EST) was used to isolate and identify a single cDNA clone from a lib rary prepared from porcine peripheral blood. Rapid amplification of cDNA en ds (RACE) was employed to clone a 1.5 kb fragment at the 5' end of the mRNA . DNA sequencing identified an open reading frame (ORF) of 2820 bp. Deduced amino acid sequence revealed the eight conserved domains characteristic of the DEAD/H box protein superfamily. The putative porcine RNA helicase indu ced by virus (RHIV-1) showed 84% amino acid similarity to human retinoic ac id-induced gene (RIG-I). Porcine RHIV-1 transcripts were ubiquitously expre ssed in various pig tissues, while in PRRSV-infected pigs, higher expressio n was observed in several tissues persistent for PRRSV. These data indicate the association of PRRSV genome replication with enhaced host cell RNA hel icase gene expression. Finally, the RHIV-1 gene was localized on porcine ch romosome 10q13 between markers SSC25A02 and SWR334 via somatic cell panel a nd radiation hybrid (RH) mapping strategies. (C) 2000 Academic Press.