Secretion of cytokines by human macrophages upon infection by pathogenic and non-pathogenic mycobacteria

The interaction of various pathogenic (Mycobacterium tuberculosis, M. avium , M. kansasii, M. xenopi), and non-pathogenic mycobacteria (M. smegmatis, M . phlei) with human macrophages at the level of macrophage cytokine express ion (TNF alpha, IL1, IL6 and GM-CSF) was investigated. Both for TNF alpha a nd GM-CSF, the lowest levels were obtained with pathogenic mycobacterial sp ecies, whereas about 2-8 times higher levels were observed for non-pathogen ic species, Contrary to the above, the differences for IL6 and IL1 were not marked, although IL6 appeared to be more elevated for non-pathogenic speci es. Heat-killed bacteria induced a lower level of the cytokines for all the three cytokines assayed (TNF alpha, IL6 and IL1), except for M. tuberculos is for whom a significantly higher proportion of TNF alpha was induced by k illed bacilli. The RT-PCR experiments performed on M. avium (as a low induc er of the cytokines) and M. smegmatis (as a high inducer of the cytokines) showed that the differences observed among pathogenic vs non-pathogenic str ains were also reflected at the transcriptional level for TNF alpha and to a lesser extent for IL6, but not for IL1. This investigation underlined imp ortant differences existing between the pathogenic and non-pathogenic speci es, particularly as regards TNF alpha and GM-CSF. (C) 2000 Academic Press.