Kvn. Rao et K. Ramaswamy, Cloning and expression of a gene encoding Sm16, an anti-inflammatory protein from Schistosoma mansoni, MOL BIOCH P, 108(1), 2000, pp. 101-108
The gene encoding Sm16, an anti-inflammatory. immunomodulatory protein pres
ent abundantly in secretions of the infective stages of Schistosoma mansoni
was cloned and partially characterized. A data base analysis showed sequen
ce homology to an earlier reported schistosomular stathmin-like gene sequen
ces reported in dbEST and Genbank. The putative gene coding for Sm16 is of
500 bp with an open reading frame of 117 aa that included an N-terminal sig
nal peptide sequence of 18 aa. There are three potential sites for phosphor
ylation (two serine and one tyrosine residue) but no glycosylation sites in
the sequence. The coding region of Sm16 was amplified from cercarial cDNA,
cloned and expressed in bacterial and insect expression systems. The purif
ied recombinant protein showed strong immunoreactivity with a polyclonal ra
bbit anti-Sm16 antibody raised against the native anti-inflammatory protein
Sm16. Contrary to earlier report, this gene appears to be not stage-specif
ic. Metabolic labeling studies suggested that Sm16 is phosphorylated and is
synthesized by both cercariae and schistosomula of S. mansoni. Sequence ho
mology with human stathmin, a cell cycle regulatory phospho protein, was 30
%. However, when probed with specific antibodies, no cross reactivity was o
bserved between Sm16 and human stathmin. (C) 2000 Elsevier Science B.V. All
rights reserved.