Cloning and expression of a gene encoding Sm16, an anti-inflammatory protein from Schistosoma mansoni

Citation
Kvn. Rao et K. Ramaswamy, Cloning and expression of a gene encoding Sm16, an anti-inflammatory protein from Schistosoma mansoni, MOL BIOCH P, 108(1), 2000, pp. 101-108
Citations number
18
Categorie Soggetti
Microbiology
Journal title
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
ISSN journal
01666851 → ACNP
Volume
108
Issue
1
Year of publication
2000
Pages
101 - 108
Database
ISI
SICI code
0166-6851(20000430)108:1<101:CAEOAG>2.0.ZU;2-9
Abstract
The gene encoding Sm16, an anti-inflammatory. immunomodulatory protein pres ent abundantly in secretions of the infective stages of Schistosoma mansoni was cloned and partially characterized. A data base analysis showed sequen ce homology to an earlier reported schistosomular stathmin-like gene sequen ces reported in dbEST and Genbank. The putative gene coding for Sm16 is of 500 bp with an open reading frame of 117 aa that included an N-terminal sig nal peptide sequence of 18 aa. There are three potential sites for phosphor ylation (two serine and one tyrosine residue) but no glycosylation sites in the sequence. The coding region of Sm16 was amplified from cercarial cDNA, cloned and expressed in bacterial and insect expression systems. The purif ied recombinant protein showed strong immunoreactivity with a polyclonal ra bbit anti-Sm16 antibody raised against the native anti-inflammatory protein Sm16. Contrary to earlier report, this gene appears to be not stage-specif ic. Metabolic labeling studies suggested that Sm16 is phosphorylated and is synthesized by both cercariae and schistosomula of S. mansoni. Sequence ho mology with human stathmin, a cell cycle regulatory phospho protein, was 30 %. However, when probed with specific antibodies, no cross reactivity was o bserved between Sm16 and human stathmin. (C) 2000 Elsevier Science B.V. All rights reserved.