Axonal transport of neuronal calcium sensor-I and phosphatidylinositol 4-kinase beta in the adult rat sciatic nerve

Neuronal calcium sensor-1 (NCS-1) and its putative substrate phosphatidylin ositol 4-kinase beta (PtdIns 4-kinase beta) both indirectly regulate synapt ic vesicle exocytosis and are located in DRG neurites. In this study we hav e tested whether NCS-1 and PtdIns 4-kinase beta are transported in axons us ing the analysis of double ligation approach in the adult rat sciatic nerve . We show that NCS-1 accumulates on both the distal and proximal side of th e nerve ligation indicating that this protein undergoes bidirectional trans port in axons. In contrast, PtdIns 4-kinase beta accumulated on the distal side which suggests that it undergoes retrograde axonal transport and unlik e NCS-1 was also present in non-neuronal cells. NeuroReport 11:1453-1457 (C ) 2000 Lippincott Williams & Wilkins.