Pyridinium trifluoroacetate/N-methylimidazole as an efficient activator for oligonucleotide synthesis via the phosphoramidite method

Authors
Eleuteri, A Capaldi, DC Krotz, AH Cole, DL Ravikumar, VT
Citation
A. Eleuteri et al., Pyridinium trifluoroacetate/N-methylimidazole as an efficient activator for oligonucleotide synthesis via the phosphoramidite method, ORG PROC R, 4(3), 2000, pp. 182-189
Citations number
51
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
ORGANIC PROCESS RESEARCH & DEVELOPMENT
ISSN journal
10836160 → ACNP
Volume
4
Issue
3
Year of publication
2000
Pages
182 - 189
Database
ISI
SICI code
1083-6160(200005/06)4:3<182:PTAAEA>2.0.ZU;2-J
Abstract
A new activator is reported for coupling phosphoramidites to a free 5'-hydr oxyl group during oligonucleotide synthesis. Pyridinium trifluoroacetate/N- methyl imidazole is a remarkably efficient replacement for 1H-tetrazole in the solid-supported synthesis of oligonucleotides, This reagent is safe and inexpensive, is not moisture-sensitive, and is soluble in acetonitrile.